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Exact(7)
Among the mapped SOLiD reads, 82.6 million (57% of mapped) were mapped in good pairs, and 37.3 million reads (26% of mapped) were mapped as single ends, i.e., the other read of the pair was not mapped.
Landslides were mapped as single points using Google Earth®, and were attributed a level of mapping accuracy P, in four classes (P1 < 1 km2, P10 < 10 km2, P100 < 100 km2, P300 < 300 km2).
Several QTLs have been mapped as single Mendelian factors (Yano et al. 2001; Lin et al. 2002, 2003; Doi et al. 2004), and map-based cloning of the underlying genes has pinpointed the nucleotide polymorphisms responsible for phenotypic variation (Yano et al. 2000; Takahashi et al. 2001; Kojima et al. 2002; Doi et al. 2004; Xue et al. 2008).
Indeed, both of the major indel events can be mapped as single events on the inhibin α-subunit phylogeny (Figs.
The qcr1 and qcr4 were precisely mapped as single QTL, using progenies BC5F1 and BC2F1, respectively.
The sequences that did not assemble in contigs were further mapped as single reads to the different genomes and less than 5% of these reads could not be mapped.
Similar(53)
In the case of the Direct mode, reads were mapped as single-end to sequences from GENCODE version 19.
Rarely, the genes were fully matched to other RefSeq proteins mapped as single-exon genes (Supplementary Fig. S4B).
All reads that passed through the cleaning step above were mapped as single-end reads using Bowtie version 0.12.7 (Langmead et al. 2009) to the B73 v2 reference sequence (Schnable et al. 2009).
Illumina 1.8 datasets were uploaded to the Galaxy website (https://main.g2.bx.psu.edu/root), FASTQ groomed (Blankenberg et al., 2010), trimmed to remove 1 nucleotide from each sequence read end, and mapped as single-end reads against the C. elegans reference genome WS220 (http://www.wormbase.org) using Bowtie for Illumina (Langmead et al., 2009).
Reads were simultaneously mapped as single-end reads to both the B73 v2 reference sequence as well as our Mo17 reference sequence using Bowtie version 0.12.7 (Langmead et al. 2009) requiring a perfect match (-v 0) and a unique alignment (-m 1).
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