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Importantly, we mapped and ordered six LMW-GS genes within the Glu-D3 locus (compared to only one at the Glu-D3 of the 1D composite map, see above), which provides a useful basis for investigating potential functional differences among the different Glu-D3 LMW-GS genes in the future.
A two-stage strategy was employed to identify genes associated with the mapped and ordered RAD-Seq SNPs.
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In conclusion, POPSEQ methodology can be used as an alternative method of mapping and ordering GBS markers.
Based on the F12 genotyping results, microsatellite markers used for fine mapping Tir2 and Tir3 loci on MMU1 and MMU5 were mapped and best ordered on each chromosome, using the MapMaker/QTL program [ 25].
Biallelic GBS markers that passed the latter test were then incorporated into a high density, framework map and ordered according to their positions in the reference genome.
Additional file 4: Table S3 provides a list of 1,332 unique co-dominant GBS markers used for map construction and ordered according to their map location on the GPMx population.
A French commercial court has found Google guilty of abusing its dominant position through its Google Maps application, and ordered it to pay a fine and damages to a French mapping company.
All other parameters applied in building the maps and ordering the markers used the default settings.
The advantage of using the POPSEQ approach for mapping and ordering GBS markers is twofold.
Fluorescence in situ hybridisation (FISH) has been used increasingly for gene mapping and ordering probes on interphase and metaphase preparations.
The results indicated that there is strong agreement between the independent de novo linkage map and the POPSEQ mapping approach in mapping and ordering GBS markers for hexaploid wheat.
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