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Sequence reads were mapped against reference sequence B73 AGP_v2 using BWA (version 0.7.5) [ 62].
Among these reads, 1.69 % (range 1.45 1.92 %) were marked as duplications and excluded; and 96.7 % (range 96.3 96.9 %) were mapped against reference genome.
The resulting reads were mapped against reference sequences from Repbase, a database containing consensus sequences for known repetitive elements (Jurka et al., 2005).
Sequences were mapped against reference sequences for splice variants AID-FL, AID-ΔE4, AID-ΔE4a, and AID-ivs3 and uniquely mapping sequences were quantified using CLC Software (Version 6.0.1; performed at GATC Biotech, Germany).
Nearly 130 million of Illumina reads (2 × 100 bp) were cleaned using prinseq (Schmieder and Edwards 2011) and mapped against reference TR-GAG sequences using bowtie 2 (Langmead and Salzberg 2012).
To determine genetic variations at the CPS loci, the reads were mapped against reference sequences for each serotype in order to obtain fine mapping using Burrows-Wheeler Aligner [ 18, 19].
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If a reference genome is available, reads of the contrasting bulks can directly be mapped against the reference sequence as described in the next paragraph.
If a high-quality reference sequence is available, as in the case of the human mitochondrial genome, the sequencing reads can be mapped against this reference.
EST data generated from 14 apple genotypes were downloaded from NCBI and mapped against a reference EST assembly to identify Single Nucleotide Polymorphisms (SNPs).
The reads were first mapped against the reference sequence for the genome of the F11 South African strain.
After trimming the reads for low quality bases, reads are mapped against a reference genome with the Burrows-Wheeler Alignment Tool (bwa [15]).
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