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Moreover, by employing this map we refined QTL to regions of 1.4 and 0.2 cM (or 400 and 50 kb) in linkage groups 2 and 3 in a population containing 380 progeny; potential candidate genes for growth traits in QTL regions were further identified using comparative genome analysis, whose effects on growth traits were investigated.
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Besides the Rfs2/Rhg1 region, we refined the mapping location with other significant SNPs within or adjacent to previously reported QTLs (Table 2).
For conversion events that had a breakpoint in a region sparsely represented by oligonucleotides on the microarray, we refined the mapping using a different method (Lee et al. 2009).
We refined the "A" map by adding the new RAPD and SSR loci.
To identify potential areas of influenza reassortment, we refined the co-occurrence maps by also incorporating swine density.
We identify two time-independent difference maps, from which we refine the structures of the corresponding intermediates.
We constructed a high resolution linkage map, carried out comparative mapping, refined the positions of QTL, identified candidate genes for growth traits and analyzed their effects on growth.
Curves are shown for model versus summed map (FSCfull), model refined in half-map 1 versus half-map 1 (FSCwork) and model refined in half-map 1 versus half-map 2 (FSCfree).
Subsequently, we computed map distances and refined the locus order with other purpose-built perl scripts.
Consensus maps were further refined by mapping single-molecule maps to consensus maps, and label positions were recalculated.
The native model was rebuilt in COOT according to 2FOFC and 1FOFC map and refined at 1.60 Å resolution against the wild-type SaTRIC native data with phenix.refine50.refine50
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