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The resulting map was filtered to 40 Å and used for 3D classification in RELION.
The final map was filtered according to local resolutions (Cardone et al., 2013).
The resulting map was filtered to 40 Å and used as an initial model for 3D classification in RELION (Scheres, 2012).
The resulting map was filtered to 50 Å and used as a starting model for an initial refinement in SPIDER (Frank et al., 1996) to regain linker density at a position derived solely from the data.
In (E – H ), the map was filtered at 4.5-Å resolution and sharpened using a B-factor of -54 Å. DOI: http://dx.doi.org/10.7554/eLife.00218.004 The large subunit FrhA has considerable homology to the known large subunit structures, and is characterized by four long α-helices.
Similar(55)
Before a final mapping preliminary mappings were filtered by in-house programs to create a final collection of reads, under the following criteria: 1. Reads not uniquely mapped were discarded.
After that, high-quality SLAF markers for the genetic mapping were filtered by the following criteria.
The final 3D density maps were filtered to 35 Å resolution as suggested by Fourier shell correlation (FSC) analysis.
To reduce noise, the attenuation maps were filtered using a 3-D gaussian kernel (FWHM, 2.5 pixels).
All detected eQTLs are presented in Additional file 2. SNPs used for eQTL mapping were filtered based on a high MAF (<0.05).
Although both sets of density maps were filtered to around 6 Å, and the maps are presented at different threshold levels, it does appear that Shang et al do have sufficiently better resolution to justify their confidence in interpreting some important loop conformations.
Related(20)
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map was screened
map was available
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map was produced
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map was generated
map was studded
map was drawn
map was swathed
map was modified
map was outlined
map was divided
map was developed
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