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Focusing on RpII140, we used in situ hybridization to map this gene to a small subinterval defined by the endpoints of a series of deficiencies impinging on the 88A/B region and showed that it does not represent a previously known genetic locus.
Here, we precisely map this gene to linkage group 8.
The polymorphisms detected in our parents allow us to map this gene in the QTL detected on chromosome 3 that we named Rg-3.
In order to precisely map this gene, we determined the nitrogen-fixing genotype of 73 F2 plants of the mapping population by inoculating their F3 progeny.
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This could be attributable to the use of a heterologous probe in mapping this gene family.
Based on the location of similar sequences in the rice genome (Genbank AP005781 and AP005802) and a comparative grass genome map [70], this gene copy is assumed to be on the Triticeae group 5 homoeologous chromosomes.
chinensis mapping population and genetic mapping linked this gene to linkage group 10.
These data were then normalized to reads per kilobase of exon region in a given gene per million mapped reads (RPKM) values, which were calculated based on the length of the gene and read count mapped to this gene.
RPKM of each gene was calculated based on the length of the gene and reads count mapped to this gene, considering the effect of sequencing depth and gene length for the reads count at the same time.
And then FPKM (Fragments per kilobase of transcript sequence per millions) of each gene was calculated based on the length of the gene and reads count mapped to this gene.
Nevertheless, the only SNP mapped within this gene could not be considered as an outlier both when considering distribution of FST across and within breeds.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com