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This structure provided valuable information about the enzyme functioning and a framework to map the mutations of the AGT gene.
As the SAAPdb was unavailable for maintenance we were unable to map the mutations in the protein structure.
To map the mutations responsible for altered sensitivity to Smex, mutants of interest were crossed to ecotype Landsberg erecta.
We then used BSA-seq to map the mutations responsible for three ethyl methanesulfonate−induced mutant phenotypes in Saccharomyces cerevisiae.
Both alleles were lethal over several deficiencies and we could map the mutations to the CG11188 locus as described in more details below.
DOI: http://dx.doi.org/10.7554/eLife.06306.006 Meiotic recombination mapping and RFLP analysis (Berger et al., 2001) were used to map the mutations responsible for the dPob-like phenotype of 008J and 655G.
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Once we identify the mutant flies, we map the mutation and characterize the mutated gene at the molecular level.
This document contains supplementary text about the multilevel map, the mutation data, the significantly mutated and altered copy number genes, and the randomly rewired map.
These 200 F2 plants were used for genotyping to map the mutation.
The mutant NH219 was crossed with another moderately heat tolerant variety IR64 (Khush and Virk 2005 Jagadish et al. 2010b) to map the mutation causing the drought and heat tolerant phenotype in the mutant and heat tolerance associate traits.
First, we tested this technique by using it to map the mutation in glp-4 nematodes, which fail to develop a germline at 25°C.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com