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Consistent with this proposal, we show that TBX5 and TBX20 can physically interact and map the interaction domains, and we show a cellular interaction for the two proteins in cardiac development, thus providing the first evidence for direct interaction between members of the T-box gene family.
We used glutathione S-transferase pull-down assays and nuclear magnetic resonance (NMR) experiments to demonstrate that BIN1 and Tau proteins interact directly and then map the interaction between BIN1's SH3 domain and Tau's proline-rich domain (PRD).
To map the interaction domains for each protein, various GFP-PTEN and Flag-DAXX deletion constructs were co-transfected with full length Flag-DAXX or GFP-PTEN, respectively.
To test whether recognition of the AATTY motif is required for GR-κBRE binding in solution, we performed nuclear magnetic resonance (NMR) footprinting analysis to map the interaction between GR DBD and the IL8 κBRE (Fig. 5a-e).
A methodology based on real space vortex image analysis is presented able to estimate semi-quantitatively the relevant energy densities of an arbitrary array of vortices, map the interaction energy distributions and evaluate the pinning energy associated to particular defects.
Considering its simplicity in interpretation, predictive accuracy, its data-driven and adaptive nature plus the ability to map the interaction between variables, the use of MARS_LR model in evaluating stability of underground entry-type excavations is promising.
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Nilsson, M., Griggs, D. & Visbeck, M. Policy: map the interactions between Sustainable Development Goals.
Purdue, meanwhile, looks to carry out techniques used to map the interactions between people in social networks into the biological realm.
A hybrid mobility law for glide and climb is used to map the interactions of dislocations with γ′ cubes.
Interactomes are global networks that map the interactions (or "links") between network nodes (proteins and other biological actors such as genes, enhancer regions, cofactors etc).
To map the interactions within the Wss1/Doa1/Cdc48 complex, we performed a series of pull-down experiments with purified protein domains.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com