Sentence examples for map the insertion from inspiring English sources

Thirty-one mutants displayed reproducible biofilm formation defects and arbitrary PCR and sequencing was used to map the insertion location (Table 1).

The iPCR method is sufficient to map the insertion site for most transposable elements, and has been used in high-throughput screens to isolate the insertion sites for thousands of P-element and piggyBac transposable elements (e.g. Ref. [7]).

This technique was originally developed to amplify the genomic DNA between a known restriction site and a target gene [9], and then adapted to map the insertion sites of viral integrating gene traps in the mouse genome [10].

Multiple attempts to map the insertion in line 688A were unsuccessful, but two independent iPCR experiments mapped line 168 to an insertion site in the krasavietz gene.

We expected to identify this duplication in those cases where we were able to map the insertion site of the IS 6110 element on both termini (73.1%).

TA-cloning and sequencing of the knockout PCR product confirmed targeting of the mouse Mena gene and allowed to map the insertion of the gene trap vector 682 bp downstream of Mena exon 2. VASP-deficient mice have been described previously [ 16] and were mated to MenaGT/GT mice to generate Mena/VASP double-deficient animals (MenaGT/GTVASP−/−).

To demonstrate the utility of spPCR, we have mapped the insertion sites for enhancer traps located within natural transposons, one of which highlights a master regulatory region for expression in a population of olfactory neurons.

Notably, of the 11 lines for which we have mapped the insertion site of the P element, none map to the known core polarity genes, so at least a subset of these insertion lines represent pathways that are not core polarity genes, but instead are rotation- or fate-specific.

Using an array-based method (Gabriel et al. 2006), we mapped the insertion upstream of 610.13, the ortholog of OPT1.

We also mapped the insertion sites of original strains with a tm234 tm246-background, and confirmed compatm234 tm246-background(dand not shown).

In the present work we mapped the insertion points of IS 6110 in all the Beijing strains available in the literature and in the DNA sequence databases.