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The second piece of information necessary to locate the beneficial mutation was to map the inserted mini-transposon.
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The MAQ program [24] was used to map the inserts of 21 to 24 bases (with a mean of 1.8 million sequences per sample) with a maximum set at 2 mismatches on the pig and viral reference sequences.
The red rectangle in the inserted map represents the studied area.
Fig. 8 Vertical cross sections of seismic structures along the volcanic front (A A′ in the inserted map).
Left and right sections are displayed along the red and blue lines in the inserted map at the upper right, respectively.
The obtained sequences were used to make a Blast search of the P. aeruginosa PAO1 genome (www.pseudomonas.com) in order to map the position of the inserted transposon.
The inserted maps show the ship track positions and precipitation occurrence for rain, snow and mixed-phase together with true-zeros (grey).
This phenomenon can also be confirmed from the inserted maps in Fig. 7a.
The adsorption phenomenon also can be seen from the inserted maps in Fig. 7a, where the equilibrium snapshots of oil and water molecules on the neutral silicon dioxide surface are displayed.
Gene mapping experiments revealed that the inserted transgene caused a 70-kb deletion in intron 3 of the metabotropic glutamate receptor 1 (grm1), which leads to an ectopic expression of this receptor.
The resultant subarray indicates the best portion of the alignment that maps to the reference or the inserted sequence.
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