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Ensemble approaches are particularly well adapted to map the conformational energy landscape sampled by the protein at atomic resolution.
Corresponding collision cross section (CCS) measurements were compiled for significant ion mobility peak features observed at each charge state in order to map the conformational landscapes of these proteins in both helium and nitrogen drift gases.
However, the peptide library constructed in this study was enriched at 100 500 bp (or less than 170 aa), which was likely insufficient to map the conformational epitopes.
Thus, taken together, the existing structural and thermodynamic data map the conformational transition from the open to closed states.
In order to map the conformational epitopes of mAb b12 13 onto the crystal structure of gp120 (PDB id: 2NY7) we selected nineteen 12-mer cysteine looped peptides.
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DOI: http://dx.doi.org/10.7554/eLife.07574.027 Here, we have mapped the conformational landscape of the dynamic enzyme CypA by analyzing multiconformer models from multitemperature crystallography.
The bioinformatics prediction (48) also mapped the conformational epitopes of annexin A2 to the C-terminus (Supplementary Table S8, available at Carcinogenesis Online).
By comparing RF1 fitted to our RC-RF1 and RC-RF1RF3 maps, the conformational change in domain 1 of RF1 upon RF3 binding can be clearly visualized.
We also mapped the conformational epitopes of mAb 17b onto the crystal structure of gp120 36 using eleven 12-mer cysteine looped peptides of the mAb 17b.
Thank you for sending your work entitled "Mapping the Conformational Landscape of a Dynamic Enzyme by Multitemperature and XFEL Crystallography" for consideration at eLife.
Mapping the conformational motion that transduces the energy of ATP binding and hydrolysis in the NBDs to the mechanical work of substrate translocation in the TMDs is central to understanding the mechanism of ABC transporters.
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