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The team used a technique called Spatial Point Pattern Analysis to map precisely the location of each of the many artifacts and biological materials.
To fully understand the biological function of a disulfide-containing protein and its regulation, it is necessary to map precisely the position of each disulfide bond and to determine the redox state of the two cysteine residues involved in the disulfide form or as free thiols, or else under conditions studied.
Probably the biggest limiting step in understanding the genetic and developmental bases of phenotypic differences lies in having enough phenotypic resolution to map precisely the underlying genetic differences that generate those differences and then being able to analyze them functionally.
Our localization studies do not map precisely the position of MEI-S332 with respect to the centromere, kinetochore, or centric heterochromatin in anaphase II cells, although they clearly show it is not present along the arms unless at very low levels.
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While we have not mapped precisely the sequences responsible for the effects described in this paper, there are very significant differences in the N-terminal regions of Hoxa13 and Hoxd13 that could explain why in the control of ingression, of elongation and of Wnt signaling, these 2 proteins show different phenotypes (see alignment in Author response image 1).
The focus for the GMS, as its name implies, is 'state', specifically, symptoms present in the previous 1 month, and although the GMS does not map precisely onto the DSM or ICD categories, it was calibrated for the detection of disorders of clinical severity, and it has been used in several studies to describe generalised anxiety disorder.
Hoonhout et al 's study hypothecated costs on categories of adverse events, but the categories he used did not map precisely onto the categories we used (based on the Harvard Medical Practice Study).
We also analyzed ESTs from E. granulosus, which are however more difficult to map precisely given the draft status of the genomic assembly.
Perhaps more surprisingly, the profile of the PRC1/2 components over CGI-associated target genes did not map precisely to the gene TSS but instead tracked with KDM2B binding and the underlying non-methylated DNA signal at these same regions.
It is possible that a newly identified class of small ncRNAs, the splice site RNAs, whose 3' ends map precisely to the splice donor site of internal exons in animals, serve as markers [ 126].
Similarly, standard FISH methods are time consuming, costly and suffer the significant limitation that some patients with uniquely localized microdeletions or duplications may yield normal clinical FISH findings because the probe set used does not map precisely to the entire region of deletions/duplication.
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