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Berg et al. [33] characterized this FAT1 QTL and refined its map position to a 3.3 cM interval between the RXRG and SDHC genes.
For the remaining 16 markers, the median distance was calculated from the marker's map position to the respective gene in the genome sequence.
Šimková et al. (2008) mapped 162 SNP loci, including 40 loci with hitherto unknown map position to barley chromosome 1H using a pilot oligonucleotide pool assay.
For the purpose of QTL analysis, clusters of markers were removed to leave only one marker at each locus (taken as the map position to one decimal place).
We then used DNA extracted from single embryos and single nucleotide polymorphism (SNP) markers to refine the map position to a 0.36 cM interval between the markers ENSDART109865 and wu:f63d09 (Fig. 3A).
Harhay and Keele [ 31] used GO and GO-annotated human sequence to link livestock EST with function; mapping the EST can extend their procedures to relate map position to function.
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'Redundant' markers (those with identical map positions to their respective framework markers) and lower quality 'attached' markers (those with less well-supported map positions) were integrated into the most appropriate positions in framework marker map.
We eliminated 162 loci because they had identical map positions to other loci (n = 151) or did not map to any LG (n = 11).
The high homology between FLcDNAs (27%) and mapped barley expressed sequence tag enabled assigning linkage map positions to 151 233 FLcDNAs on each of the seven barley chromosomes.
Connecting map positions to GO terms requires synchronizing several information sources, including livestock maps, human sequence annotated GO terms, and GO databases.
Sato et al. (submitted for publication) assigned linkage map positions to 2890 non-redundant 3′ ESTs, providing the densest, reliable barley map available.
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