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Where the cDNA shows up, as determined by a test that makes millions of copies of the representative material, suggests the proper map position for the cDNA and hence the probable location of that gene.
Marker selection and map position for loci on chromosomes 1 and X are provided in Table 1 >.
Similarly, out of 438 NB-LRR genes, physical map position for 370 (84%) genes was predicted on potato chromosomes.
In order to solve the correct map position for these ambiguous loci we examined the GenCall ratios as discussed above (Table 2).
No framework mapping adjustment was carried out in this study as the main objective was to provide the most likely map position for all microsatellite markers reported.
Using the oat × maize radiation hybrids we were able to validate the map position for 19/20 of these genes to chromosome 5S [see Additional file 3].
Similar(46)
However, we validated map positions for 25 mapped features chosen at random using the oat × maize chromosome addition lines [ 18] and radiation hybrid oat × maize lines [ 21] and found that 24 of the 25 features were correctly mapped to the proper chromosome region using our computational approach.
Map positions for arrayed cDNA clones were assigned using the NCBI genome assembly, accessed through the UCSC genome browser database (NCBI Build 36.1).
We have been able to obtain map positions for each mutant using F2 mapping populations.
The final two SNPs provided map positions for gmw1-57d24 and gmw1-27d20 (Table 1).
Map positions for the cluster were assigned by Blat searches against the "Golden Path" genome assembly (; June , 2002Freeze).
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