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An accurate and saturated genetic linkage map of soybean is an essential tool for studies on modern soybean genomics.
We generated a high-density genetic linkage map of soybean using expressed sequence tag (EST -derived microsatEST -derivedrs.
An EST, bacterial artificial chromosome (BAC) end sequence and marker amplicon sequence synteny map of soybean and A. thaliana is presented.
Among 880 SSRs, 789 SSRs were from the integrated linkage map of soybean (Song et al. 2004) and 101 were newly developed markers (Song et al. 2010).
For instance, we tested 890 SSRs, mostly from the integrated linkage map of soybean (Song et al. 2004) for polymorphism between the parental inbred lines ZH and ZP.
Xia et al. [ 16] constructed an integrated, high-density linkage map of soybean using RFLPs, SSRs, sequence-tagged sites (STSs), and AFLP markers.
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Thus, our results will likely be useful for marker-assisted breeding and sequence-level gene mapping of soybean.
Connections could be made between the linkage maps of soybean and L. japonicus using the positional information on ESTs, as well as matched genomic clones (Fig. 3).
The syntenic blocks found between common bean and soybean Gm10-B07, Gm5-B023, Gm18-B01 Gm19/Gm15/Gm12-B06, Gm18-B01, Gm3-B10, Gm12/Gm11-B11 and Gm6-b09 were consistent with the RFLP based comparisons of the linkage maps of soybean, common bean and Vigna radiata [ 45].
Two high density genetic linkage maps of soybean based on 21,478 SNP loci mapped in the G. max x G. soja population with 1083 recombinant inbred lines and 11,922 SNP loci mapped in the G. max x G. max population with 922 RILs were constructed.
Hisano et al. used microsatellite markers obtained from the public EST databases to generate a high-density linkage map of the soybean genome.
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