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After comparing the physical and genetic map of pig genome, the reported QTLs in the genome were obtained by interpolating their linkage map position via anchor markers (details in [ 34]), we assigned annotated genes to these QTLs, producing 4,055 QTL-candidate genes.
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The brain 72,000 Mr fragment was not derived from contaminating erythrocytes since peptide maps of pig brain and pig erythrocyte 72,000 Mr fragments were distinct.
These two panels have been used extensively for the physical mapping of pig genes and markers prior to the availability of the pig genome sequence.
Using whole-genome assembly comparison (WGAC) and whole-genome shotgun sequence detection (WSSD) approaches by next generation sequencing (NGS), we probed formation signatures of both segmental duplications (SDs) and individualized CNVs in an integrated fashion, building the finest resolution CNV and SD maps of pigs so far.
The availability of such information provides the basis for construction of the genome-scale regulatory networking map of the pig miRNAome.
This genome sequencing and assembly effort is being performed under the direction of the international Swine Genome Sequencing Consortium [11] using a hierarchical shotgun sequencing strategy in which BAC clones from a minimum tiling path of clones are selected from the highly contiguous physical map of the pig genome [18].
Moreover, a highly continuous BAC map of the pig genome has been developed [ 13].
Each QTL is reported as an interval on the genetic map of the pig genome.
Besides the SD map of the pig reference genome, we also constructed a CNV picture involving 3,131 unique regions using WSSD through re-sequencing 13 highly representative individuals from ten distinct breeds or populations.
We describe here genome-wide high resolution RH maps of the pig autosomes constructed by genotyping the two pig RH panels with the Illumina porcineSNP60 array.
Among the 17,183 contigs, 16,121 were mapped on pig chromosomes.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com