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A total of 151 genic markers were used to generate the first gene-based and comparative map of faba bean.
The first genetic map of faba bean composed exclusively with gene-based co-dominant molecular markers was constructed using a F6 RIL population between lines Vf6 and Vf27.
The most recent maps of three RIL mapping populations,Vf6 × Vf27 (RIL1), Vf6 × Vf136 (RIL 2) and 29H × Vf136 (RIL3), were used to develop an integrated map of faba bean (Table 1).
A gene-based genetic map of faba bean genome was constructed using a population of 94 F6 RILs generated from a cross between faba bean line Vf6 (equina type) as the pollen recipient and line Vf27 as pollen donor (paucijuga type).
In this study orthologous codominant cross-species markers have been deployed to produce the first exclusively gene-based genetic linkage map of faba bean (Vicia faba), using an F6 population developed from a cross between the lines Vf6 (equina type) and Vf27 (paucijuga type).
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Grid Map [ 26] was used to compare the genetic maps of faba bean and M. truncatula.
One of the first genetic linkage maps of faba bean was constructed by Van de Ven et al. [ 4] with only 17 markers; followed by successively more detailed genetic maps by Torres et al. [ 5] with 51 markers, Satovic et al. [ 6] with 157 markers, Vaz Patto et al. [ 7] with 116 markers, Roman et al. [ 8] with 121 markers, Roman et al. [ 9] with 192 markers and Avila et al. [ 10] with 103 markers.
Three sets of faba bean mapping data were used in the construction of a consensus map connecting information of 11 F2 populations, marker data of 3 RILs, as well as new markers genotyped in the present study (Tables 1 and 2).
Formal genetic analysis of faba bean, such as through genetic linkage mapping and identification of quantitative trait loci (QTLs), has so far been hindered by these aspects of biology [ 19].
The objective of this study was to construct a reference consensus map in faba bean by joining the information from the most relevant maps reported so far in this crop.
Although FabA was not mapped in the aforementioned study, it was suggested that under phenol stress, the expression level of FabA correlated with the upregulation of FabB.
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