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Herein, we reported a high-density genetic map for soybean.
Herein we report a high-density genetic map for soybean, which is constructed based on large-scale markers developed by specific length amplified fragment sequencing (SLAF-seq).
The resulting linkage groups (LGs) were reorganized with reference to the integrated genetic map and a previously reported map for soybean.
Among such molecular markers, SSRs in particular have contributed to the construction of a genome-wide linkage map for soybean with a converged linkage group (LG) number equal to the chromosome number.
Recently, Hyten et al. developed a high-density integrated genetic linkage map for soybean, the Consensus Map 4.0, by compositing the SNP loci data obtained from five mapping populations [ 11].
In order to construct a genetic linkage map for soybean, we used a mapping population of 94 individual plants, which represented the F8 generation of recombinant inbred lines (RILs) derived from an F2 population generated between the parent plants 'Misuzudaizu' and 'Moshidou Gong 503'.
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A limitation when utilizing a model legume and comparative mapping for soybean marker development was reported by Hwang et al [ 20].
Despite this, the ease of phenotyping has resulted in the inclusion of this locus in the development of genetic maps for soybean [ 25- 27].
Despite the inclusion of this phenotypic marker into the extensive genetic maps developed for soybean over the last twenty years, the genetic basis behind the phenomenon of brown seed coats (the R locus) has remained undetermined until now.
An accurate and saturated genetic linkage map of soybean is essential for studies on modern soybean genomics, i.e. identification of subtle or new trait loci including quantitative trait loci (QTLs), map-based cloning, and physical map construction or even whole-genome sequencing.
For instance, we tested 890 SSRs, mostly from the integrated linkage map of soybean (Song et al. 2004) for polymorphism between the parental inbred lines ZH and ZP.
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