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The elemental distribution map for sample 2 was collected in the same manner as sample 1, except the beam size was 10 × 10 μm and step size was 7 μm.
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Figure 4 AFM images and C - AFM current map for samples annealed without a protective carbon capping layer.
Figure 3 AFM images and C-AFM current map for samples annealed with a protective carbon capping layer.
Details on read mapping for sample FD1.
Details on read mapping for sample DTT1.
Details on read mapping for sample CsCl1.
We obtained 314.2 million raw reads on a single lane, after barcode mapping for sample allocation we obtained 65.2 million reads for RNA-seq sample 1 and 58.8 million reads for RNA-seq sample 2 respectively.
AR FISH maps for samples 3 and 4 are shown in Figure 4A and B. For sample 4, we detected small regions (foci) of cells with Cat5 and Cat4 alterations: both of these foci had been selected in the TMA cores (Table 3).
This process was repeated for eight iterations, yielding a high quality consensus optical map for that sample.
Variants were detected by first constructing an optical consensus map for each sample using an iterative assembly strategy initially guided by an in silico map of the human reference.
Table 2 Results from stepwise linear regressions of the different forms of carbon densities and soil temperature (ST), soil moisture (SM), soil clay fraction (Clay), mean annual air temperature (MAAT), and mean annual precipitation (MAP) for 18 sampling sites.
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