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Compared to the previous lotus genetic maps, the American lotus map presented here has a longer map distance, and higher marker density, with around 20 folds more markers.

The non-linear regression of the LD measure (r) relative to genetic map distance and the genetic distance at which the estimated r fell below 0.2, as well as the effective size, were estimated (Table  6).

By contrast, there was a significant logarithmic relationship between the mean difference of observed and expected map distance, and the length of the genomic interval (map distance) in the coevolved lines.

After 11 generations of experimental coevolution, the mean difference between observed and expected Kosambi's map distance in the control lines (without parasites) did not relate to the expected map distance and did not significantly deviate from the line of no difference.

To be able to compare the slopes of the regression lines for the relationship between the mean relative difference between observed and expected map distance and the expected map distance of the control and the coevolution treatment, all expected map distances were ln-transformed.

Similar(55)

(See Additional file 'Genetic Map Distances' and Additional file 'Genotypes' accessible at Dryad Digital Repository).

Subsequently, we computed map distances and refined the locus order with other purpose-built perl scripts.

Here, the gross asymmetry and partial dominance of viability selection is clearly associated with inflation of ML map distances and incongruence of RG and ML marker orders.

However, the map distances and marker orders of the common SSR markers differed, possibly due to the intraspecific nature of our mapping population.

Slate et al. [ 58] demonstrated that, when genotyping error is present, markers tend to be assigned to correct chromosomes but with inflated map distances and order errors.

Apart from higher LOD scores values, no significant modifications were found in marker order or in map distances, and the number of unlinked markers was unchanged.

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