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Fourth column: motion saliency map calculated with HOAOF.
Third column: motion saliency map calculated with optical flow directly.
The model of EGF3 was manually built into an electron density map calculated with the partial structure containing LNS5 and LNS6.
An electron density map calculated with phases from the model after a single round of positional and B-factor refinement revealed clear density for the UDP-Ara4N-PLP aldimine in the active site.
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As a final test, the TF maps calculated with the +10% attenuation coefficient maps were also rotated anticlockwise by 15° and shifted by 3 mm in the x direction.
The binding of H4B cofactors was detected by the initial difference Fourier maps calculated with REFMAC.
Binding of inhibitors was detected by the initial difference Fourier maps calculated with REFMAC.
Segregation analyses were performed in the East Lansing chicken reference back-cross mapping population [ 17], and genetic localizations on the maps calculated with the Mapmanager software [ 35].
Cycles of manual building/rebuilding using Coot [ 25] were alternated with rounds of refinement employing REFMAC [ 24], plus composite omit maps calculated with CNS [ 26].
A water molecule (at the expected position of the cyano group) was introduced by a peak search of the Fo − Fc maps calculated with the benzaldehyde omitted.
Therefore, the interval analysis was done using marker maps calculated with an expansion factor of 2, 3 and 4 to check the sensitivity of the results to this parameter.
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