Moreover, while NleH1 binds NHERF2 only, EspI and Map bind both NHERF1 and NHERF2.
Moreover, via its C-terminus, which comprises a class I PSD-95/Disc Large/ZO-1 (PDZ -binding motif (TRL), MaPDZ -binding1 of Na+/H+ exchanger regulatory factor 1 (NHERF1) (Alto et al., 2006; Simotif eTRLl., 2006).
Microtubule-associated proteins (MAPs) bind to the tubulin subunits that form microtubules.
In addition, Map binds PDZ1 of NHERF1.
Overlaying a fusion consisting of the last 50 amino acids of Map and GST (GST-MapC50) on the PDZ-domain array confirmed that Map binds PDZ1 of NHERF1 (Fig. 1A).
The higher IC50 values we observed for MAP in our in vitro experiments partially reflects its lack of enzymatic activity (ETA is an ADP-ribosyl transferase that acts catalytically against its targets, whereas MAP binds stoichiometrically to microtubules).
I found the manuscript well written and very useful in general, although felt it could have considered more broadly the use of ChIP to map chromatin bound proteins or DNA modifications, beyond mapping transcription factors or histone modifications.
Microtubules in plant cells are regulated by multiple MAPs to enrich the scope of microtubule behavior, and some MAPs are bound tightly to the plasma membrane (Gardiner et al., 2001; Ambrose and Wasteneys, 2008; Gu et al., 2008; Li et al., 2011).
Different maps were bound in, depending on a captain's needs, and the handwritten table of contents rarely corresponds exactly to a volume's actual contents.
