These novel stable QTL regions are potential candidates for fine mapping and map based cloning.
Most of the genes characterized so far in model and cultivated plants have been cloned employing map based cloning approach [ 23- 26].
Moreover, combining syntenic studies with a consensus map will contribute to increase marker density in genomic regions of interest for indirect selection or for map based cloning [ 37].
Borkh (American chestnut ) to aid in the backcross breeding effort and in the eventual identification of blight resistance genes through genomic sequencing and map based cloning.
Establishment of integrated physical and genetic maps for switchgrass will accelerate mapping of value added traits useful to breeding programs and to isolate important target genes using map based cloning.
Availability of abundant markers will facilitate association mapping, marker aided selection (MAS), and fine mapping of regions of interest for circumventing the problem of linkage drag during introgressions and for map based cloning.
Establishment of integrated physical and genetic maps for switchgrass will make it possible to map value added traits useful to breeding programs and to isolate important target genes using map based cloning.
Specific-area marking and mapping of genes, as has been shown in this study for the region containing the tet-o locus, will also be useful for map based cloning.
Taking rice for example, a number of genes (or QTLs) for yield traits, including tillering [ 7], number of grains per panicle [ 8] and grain weight [ 9], have been isolated through map based cloning.
The knowledge of sequence variation and especially the availability of a high number of molecular markers for a species serve as tools for various plant genetics approaches, such as marker-assisted backcrossing or map based cloning [ 2].
Map based cloning and genetic complementation of ehy5 mutants reveal that EHY5 codes for HY1 (HO1), a rate-limiting enzyme that catalyzes the conversion of heme to biliverdin IXα (BV) in the chromophore biosynthesis pathway [ 54].
