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A cocktail of MAP assays for the common 15/18 bp deletions, together with a Bi-PAP-A assay for the common L858R mutation, could detect about 70 80% of EGFR mutations constituting about 10% of total lung cancers [10], [36].
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In addition to these two patients, four others with stage II or III breast cancer had tumor-specific p53 mutations identified and MAP assays developed for their analysis in blood (Table 2, Supplementary Fig.S5).
A duplex MAP assay for the common 15 and 18 bp deletions was performed as proof of principle of the multiplexing potential of MAP assays (data not shown).
The expandable reference map of SRM assays for CAPs is a valuable resource for designing and accelerating biomarker verification studies.
Mass spectrometric reference maps consisting of assays for targeted quantification of PTPs are established or under development such as those of plasma proteome, N-linked glycoproteome, CAPs sets and even the human global proteome.
This reference map consisting of SRM assays for 5,568 N-glycosites is publicly available via the SRMAtlas [68], which can be used for unbiased analysis in plasma biomarker research.
These evaluations demonstrated the substantial potential of the multiplexed antibody binding and mapping assay for rapid and sensitive analysis of complex antibody responses.
The effectiveness and suitability of GoldenGate ® SNP assays for genotyping mapping populations and genetic resource collections of pea has been previously demonstrated [ 18].
For the rat, MAP assays were developed for the EGFR 15 and 18 bp deletions homologous to those found in human.
To develop mAb-specific binding assays for each antitoxin, we mapped the epitopes of the six mAbs.
They are thus well suited as assays for phylogenetic analysis, the construction of genetic maps, marker-assisted breeding, transcript mapping and other genomic applications in the species.
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