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There are also regions of discontiguous alignments between the physical map and draft sequence.
Using marker sequences, the RH maps were also anchored with a BAC contig based physical map and draft sequence of the D-genome of Ae. tauschii.
Based on the alignment between optical map and draft assembly, the size difference is likely derived from 3 major sequence gaps approximately 30, 70, and 10kb in size (Additional file 1: Figure S1).
With the high-density map and draft genomic sequence of S. japonica being available [ 8], it is possible to conduct comparative analysis between U. pinnatifida and S. japonica in the next step [ 49].
Because our purpose was to pick the most reliable and complete MTP, and not the MTP with the minimal number of clones, the FPC map and draft sequence were integrated and clones with unpaired BES were added to build a high-quality physical map with the fewest gaps possible (http://soybase.org).org
To compare our pachytene FISH map directly to the genetic linkage map and draft genome assembly map, we used a standardized map-unit system in which the percentage distance of each locus along the chromosome in each map was used, denoted as RMP units.
Similar(54)
SvD constructed and analysed the co-expression map and drafted the manuscript.
EM screened the RH panel, analyzed the RH data, constructed the map and drafted the manuscript.
SL built the RH map, and drafted the manuscript with FP.
FY-L carried out genetic and molecular marker analyses, developed the genetic map and drafted the manuscript.
NT contributed to the design of the study, led the data collection and selection of criteria for inclusion in the map and drafted and finished the article.
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