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Optical map alignments of Budgerigar_v6.3 and PBcR assemblies in Microsoft Excel and XML formats and software (Gnomspace.rar) to view the XML alignments.
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In this article, we introduce a polynomial-time algorithm for optimal pairwise contact map alignment of protein interfaces using two-dimensional (2D) dynamic programming.
Map alignment of different populations (presented in Additional File 1) and dot-plots (presented in Figure 2 and Additional File 4) became powerful tools to indicate genetic regions where maker order differs among population-specific maps.
Genome alignments were performed with MAUVE [ 84], mapping alignments of homologous genes to a reference genome was conducted with GeneWiz 0.94 [ 85].
Because physical distances (in kb) between restriction sites are accurately determined by optical mapping, alignments of an " in silico" restriction map, constructed from sequence data, against our optical map reveal discordances characterized by features commonly associated with ordered restriction maps.
One important benefit of this transition has been greatly improved alignment and fine mapping of QTL relative to the underlying genes/alleles and precise inter-map alignment of QTL identified in populations derived from different parental genotypes.
The procedure to close unresolved gaps and correct any remaining sequence errors detected is described in detail in the Methods section and included the use of previously available BAC sequence information [ 14, 15, 17, 18], PCR-aided gap closing, and manual correction of the final sequence by visually inspecting the mapping-alignments of the re-sequencing reads.
Such issues became most apparent through our analysis of optical map alignments at telomeric regions, or ends of chromosomes.
Visual representation of map alignments was achieved using AutoGRAPH [ 30].
Visual representation of map alignments was achieved using cMap [ 38].
It is, however, limited to computing all pair-wise alignments, with no post-processing and visualization of map alignments.
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