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Also, according to the prepared map, a total of 23.22% of the area, amounting to 105.38 km2, is in the high and very high-risk class.
The RE-ANN calibration method was employed for the M5 model to successfully map a total of 27.43% DDE grid cells, which was smaller than that of RD2T by a small margin.
To develop the whole-genome physical map, a total of 38,208 clones from the BAC library were fingerprinted with SNaPshot HICF FPC technique [24].
Next, we were able to map a total of 62 pathways using the dataset from a study by our group in which C. difficile 630 was used to infect Caco-2 cells up to 120 min post-infection [34].
After the two-round hybridization selection (details see Materials and Methods) and comparison with the genome-wide physical contig map, a total of 5,005 positive clones were identified for fingerprinting.
For each sex-specific map, a total of 40 LGs was also obtained.
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The genetic map had a total map length of 1650 cM with an average resolution of 0.59 cM between markers.
This map has a total map distance of ∼1780 cM (in Haldane's units) and an average interval length of ∼23 cM.
The male map had a total map length of 4141.76 cM (File S3).
The consensus map spanned a total map length of 530 cM and provided the means to propose a standardised linkage group nomenclature for Rosa.
The optical map assembly resulted in six maps with a total map size 7,289 Kb, similar to the total contig length.
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