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Many transgenic lines of crop plants are commercially advantageous and are being introduced into the market.
Advancements in genetic engineering over the last two decades have introduced many transgenic lines that have been screened to study the pathomechanisms of depression.
However, the use of many transgenic lines is limited by low breeding efficiencies, high premature death, late onset and high inter-animal variability of the pathology creating a need for improved animal models addressing these questions [3], [4].
However, the use of many transgenic lines is limited by premature death, low breeding efficiencies and late onset and high inter-animal variability of the pathology, creating a need for improved animal models.
Many transgenic lines show high variability in the extent and time course of expression of disease phenotypes.
However, under salt stress conditions, many transgenic lines exhibited significantly increased height growth relative to WT plants.
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In a separate experiment, we have obtained many transgenic rice lines overexpressing maize cytosolic carbonic anhydrase (CA) (Additional file 3: Figure S3A) and one of the lines exhibited reduced tiller number whereas other lines showed a normal tiller phenotype.
Further, although many transgenic mouse lines with modifications in the Wnt signaling pathway exist, most harbor perturbations in downstream effectors, such as Apc or β-catenin.
To develop an alternative method that complements the constitutive promoter systems, we constructed RNAi and/or antisense transgenes for four rice genes using a constitutive promoter or a cognate promoter of a selected rice target gene and generated many independent transgenic lines.
Moreover, because the transgene is present as a single copy at a known site of integration it circumvents the need to generate many different transgenic lines from a given construct as is the case when using a transient transgenic procedure.
Many other transgenic lines that label either the entire myeloid population, the early myeloid subset, microglia or all antigen-presenting cells are also very useful for the study of macrophage biology (supplementary material Table S1).
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