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MicroRNAs need to be co-expressed with their targets [4] in order to be functional, and many transcripts in an organism are only produced in response to internal or external stresses.
5'-triphosphate species) of antisense siRNAs are produced against many transcripts in a two-step amplification by RNA-dependent RNA polymerases RRF-3 and RRF-1, respectively, in conjunction with DICER, Argonautes and other specificity determinants.
As both types of endoderm express many transcripts in common (Foxa2, Gata4, Gata6, Sox17, Hnf4α, Afp, Ttr,.), one should take into account the minimal differences in transcript and protein expression between these two types of endoderm when investigating in vitro differentiation of pluripotent stem cells towards hepatic endoderm.
Technologies now exist to measure the abundance of many transcripts in parallel.
NOVA crosslinks to many transcripts in both nuclear/intronic and cytoplasmic/3′ UTR clusters, suggesting an ordered set of cis-actions on target RNAs.
The method is particularly well suited for gene expression assays that involve quantitation of many transcripts in large numbers of samples.
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The presence of EGF domains in many transcripts expressed in the venom glands of all three investigated species of Glycera is probably the reason why significant BLAST matches (e value ≤ 0.00001) for gigantoxin I are found in all of our libraries.
Besides these, many transcripts involved in pathogen defence were induced/repressed in leaves and flowers of tolerant and susceptible genotypes (e.g. CV793610, DY396305, DY396390, DY475397, DY396269 and DY396359).
We interpret the strand bias as evidence that sequences intrinsic to the interspersed repeats themselves are directing much of this transcription, consistent with the observation that many transcripts originate in this portion of the genome [ 7].
Underexpression of many transcripts found in cluster B has been previously reported in several models of aging and AD as well as in AD patients.
Analysis from our in vitro and clinical experiments has demonstrated that many transcripts exist in sense:antisense pairs including IGF2BP2, which may have a direct regulatory function in the context of colorectal cancer.
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