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Strategies to achieve controlled, localized expression within the tissue engineering scaffold will have broad application to the regeneration of many tissues, with great promise for clinical therapies.
Many tissues with a branched architecture, such as the vasculature, kidney, mammary gland, lung and nervous system, function to exchange fluids, gases and information throughout the body of an organism.
Whole genome oligonucleotide arrays have been extensively used to examine mRNAs expressed in many tissues with different developmental stages or treatments.
Uncoupling protein-2 (UCP2) is present in many tissues with relevance to fuel metabolism, and its expression is increased in fat and muscle in response to elevated circulating free fatty acids resulting from fasting and high fat feeding.
In the current study, we have compared the metabolic and regulatory properties of PTG (expressed in many tissues), with two other members of the gene family, G L) (expressed primarily in liver) and G(M /R(Gl) (expressed primarily in striated muscle).
Taking it one step further, our data show that a fraction of mammalian IRE1α and PERK is constitutively active in many tissues, with ∼10% IRE1α being phosphorylated and activated.
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In physiological conditions, the level of β-defensin expression in many tissues varies with exposure to bacteria and proinflammatory cytokines.
This has shown DEC1 to be widely expressed in the nuclei of cells in many tissues, but with a restricted pattern of expression.
However, the fact that most genes are expressed in many tissues, combined with the normalization procedure probably increased the chances of picking them up in such a pooled-tissue library.
We found that MTP5, like IAR1, was widely present at similar levels across many tissues, consistent with the possibility that the MTP5 and IAR1 proteins might influence similar functions.
Aging eventually is characterized by marked atrophy of many tissues and organs, with both a decline in the number of cells and an alteration in their constitution.
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