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We chose the ROSA26 locus because it supports global expression in many tissues during mouse development [15], [16].
Klf4 and c-myc are expressed in many tissues during embryogenesis in mouse and rat and are not strictly markers of pluripotency alone [42], [43], [44].
MEG3 is expressed in many tissues during development including the central nervous system and most mesoderm derived tissues where it is co-expressed with DLK1 [26].
DLK1 is widely expressed in many tissues during mammalian embryonic development [25] [27] and is implicated as an important developmental growth factor.
In a recent study, it was reported that tamoxifen inducible Cre-ER line has toxicity in hematopoiesis and the increase of apoptosis in many tissues during embryonic development, and the toxicity also depends on the injection dose of tamoxifen [37].
In mammals, ROBO1-3 is expressed in many tissues during development and particularly in the nervous system [ 17– 17].
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Indeed, lineage branching is a common feature of many tissues, both during development and regeneration [ 21- 25].
IGF-1 regulates development and growth of many tissues, particularly during the prenatal period.
NAC is regarded as a plant-specific transcription factor family member and expressed in many tissues and during many developmental stages [ 92].
hmr-1 is expressed in many tissues and timed during development; among other places it is highly expressed in apical junctions of hypodermal cells during embryonic development and later additionally becomes localized to the apical margins of the intestine and pharynx [22], [23], [24].
Tissue-specific alterations related to age at exposure: Exposure during development may be more likely to induce widespread changes (i.e., many tissues affected), whereas exposure during adulthood may be more likely to induce restricted changes, perhaps identifiable in only one or a few tissues (Rakyan et al. 2011).
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