These experiments, however, require many technical and biological replicates to account for run-to-run variability.
Here we show that the AB platform has substantially higher sensitivity, detecting four times as many gene changes in an identical experimental design, and over seven times as many genes when additional technical replicates were included.
The AB platform identified over 3.5 times as many genes (1927 vs. 548) using equivalent replicates and analyses, and over six times as many genes (3478 vs. 548) with additional technical replicates.
To obtain many kinds of transcripts, data on nine technical replicates of the sequencing run of cDNA from the roots after salinity stress were accumulated.
Many of the early RNA-seq datasets involve sequence reads from technical replicates (e.g. same source of RNA) as opposed to biological replicates (e.g. RNA from different individuals).
Broad quantitative analyses of many biological states over several time points supported by an adequate number of biological or technical replicates can quickly become financially impractical.
Moreover variation across technical replicates is likely to be lower than that typically observed across true biological replicates, and many biological effects of interest may be smaller than two-fold [12].
In many instances, multiple (L) time series may be available, either because of multiple related initial conditions or due to biological and/or technical replicates.
Many technical tools are descriptive, not indicative.
Error bars indicating SD obtained from three technical replicates.
