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Many substrates of enzymatic reactions are soluble in unpolar organic solvents whereas the enzymes themselves often need an aqueous environment.
Many substrates of E3 ubiquitin ligases have been identified as being monoubiquitinylated.
The fact that many substrates of GSK3β are cytoskeleton-related proteins [16] prompted us to investigate the effects of GSK3β inhibition on the acetylation of tubulin.
That is why it is important to identify as many substrates of Fbs1 as possible.
Many substrates of ROCK1 have been identified, including MYPT, ERM family and LIMK.
Many substrates of PINK1 and mechanisms for PINK1-mediated Parkin activation have been postulated (Wang et al, 2011; Okatsu et al, 2012; Chen & Dorn, 2013).
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Activated Akt has many substrates including members of the forkhead box O (FOXO) class of transcription factors, which are involved in cell fate decisions, proliferation, and metabolism [ 34, 35].
Chitosan solubility, biodegradability, reactivity, and adsorption of many substrates depend on the amount of protonated amino groups in the polymeric chain and thereby on the proportion of acetylated and nonacetylated glucosamine units.
Ubiquitination and subsequent degradation of many substrates is important for driving cells through anaphase and allowing the completion of mitosis [122].
However, sumoylation of many substrates would be affected by this approach, since there exist only very limited numbers of desumoylating enzymes.
Inhibiting Ulp1 will increase the sumoylation level of many substrates in addition to Tec1.
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