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The script was originally too long, and as a result many sequences had to be cut, some even after they had been filmed.
Many sequences had no close relatives.
Still, many sequences had to be generated to meet the various design objectives.
Except the sequences with high Ka/Ks, many sequences had Ka/Ks values equal to or only slightly greater than zero, suggesting that these genes have evolved under high selective constraint (See additional file 4) [ 39].
We also performed simulations using overlapping regions of 750 sites and 300 sites, respectively (supplementary fig. S3, Supplementary Material online), based on the parameters used in figure 3 A and B. When the overlapping region is short and the distance is low, many sequences had no substitution in NS sites or SN sites, making our method inapplicable.
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SREs are more suitable for the prospective forecasts than previous studies (e.g., Nishenko, 1991), because the events are objectively identified, the occurrence times are accurate, the recurrence intervals are much shorter, and many sequences have been found.
He can easily verify how many sequences have been discarded during the alignment process and how many sequences have been aligned to the different chromosomes in the reference sequence.
Many sequences have one or more matches to known protein domains, but still contain long stretches that are not computationally characterized.
Similarly, the user can even find out how many 3' sequences have a verified polyA tail (i.e., 3TSS, 3' terminus in the sense strand) or how many 5' sequences have a verified polyT tail (i.e., 5TNS, 5' termini in the non-sense strand).
Many dream sequences had to be altered due to production issues, though series creator Eric Kripke believes it ultimately benefited the episode.
The initial sequence comparisons indicated that many LK sequences had been partially misannotated during the automated annotation process.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com