The data presented by Schindler and Waldminghaus in Figure 2A is misleading because the scales are not comparable for the two datasets, and the time-points after replication initiation are different (signal at the replication origin is expected to drop rapidly following replication initiation).
In advance of DNA replication initiation, PCNA is bound to potential start points of DNA replication and relative enzymes; DNA polymerase α and δ are essential for DNA replication in the nucleus.
Limiting levels of initiation factors have been reported [ 15, 16], and constitute a point of control of replication initiation.
Our results suggest that many viral origins may actually be located in replication initiation zones.
Many plasmid backbone genes essential for conjugative transfer, replication initiation, and active partitioning are regulated both autogenously and by host factors [ 5].
Since aberrant overexpression of the replication initiation machinery is found in many types of cancers, CDC6, CDT1, MCM2 and CDC45 may also be useful markers for assessing the type and degree of oral pre-malignant as well as malignant lesions.
Many lines of genetic and biochemical evidence suggest that Rb restricts replication initiation, although its mechanism is not clearly understood.
This points to a role of parABm in megaplasmid replication initiation and/or copy number maintenance.
However in many plasmids insertions occur in this region, separating the oriV and the replication initiation region.
The synthetic phenotype between sod1 and pri2-ts points to a connection between oxidative stress and DNA replication initiation.
Similarly, we predict frequent pause events at the 3′ end in the CRs, because the replication initiation and closure domain of N-strands exist at this domain, many replication events such as segregation of N-strand molecules and superhelical turns [ 35] occur while the replication forks are traversing this section.
