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How many million mapped reads and how many replicates were done for each sample?
Although SRM data were reported, it was not possible to determine how many replicates were processed, the corresponding measurement precision, or how many replicates were used for establishing intra and inter-assay precision.
No smoothing was performed for the day-of-week term, since many replicates were available for each day of the week.
1) Detailed information concerning the Ribo-Seq experiments is missing: How many million mapped reads and how many replicates were done for each sample?
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How many independent EVC lines should be included to test for transformation effects, and how many replicates are necessary to detect significant ecological effects of single genes?
The question 'how many replicates is enough?' is complicated by many potentially confounding factors such as the type of array equipment, laboratory technique, and, most importantly, the quality of the samples.
How many replicates are there for the deep proteome?
Its slowness makes it cumbersome when simulation with many replicates is needed.
How many replicates are needed depends on the heart rate at which optimization is to be carried out (Table 1).
The issue of how many replicates are required in a typical experimental system needs to be addressed.
Unfortunately, WGBS is still prohibitively expensive for most laboratories, particularly when many replicates are required; significantly, a high fraction of reads contain no methylated cytosines.
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