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This suggests that deep pyrosequencing is effective in revealing the expression of many rare transcripts, e.g. transcription factors.
Nevertheless, we still expect many rare transcripts to be represented as singletons alone.
Due to the sensitivity of Solexa sequencing technology, many rare transcripts were detected.
Previous transcriptome studies have suggested that many rare transcripts may be the product of biological noise, although few have provided evidence that these products are non-functional.
Not only can the 454 sequencing technology identify a large number of expressed sequences, but it can also discover new genes via deep sequencing, effectively revealing the expression of many rare transcripts.
This supports the idea that deeper EST sequencing using 454 technology will identify a larger number of expressed sequences than conventional EST sequencing and is effective in revealing the expression of many rare transcripts.
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Many other rare transcripts of this gene fusion have been described (Soller et al, 2006; Wang et al, 2006; Clark et al, 2007; Liu et al, 2007; Tu et al, 2007).
Due to the large number of reads afforded, the 454 DNA sequencing technology is effective in revealing the expression of a large number of genes and has a great potential for discovering many rare or novel transcripts [ 21] also in non-model organisms where few previous ESTs sequences are available [ 16].
Many singletons were from rare transcripts, as expected, though many more represented highly expressed transcripts.
Increasingly sensitive sequencing technologies will reveal rare transcripts but many of these are clearly non-productive.
Many of these additional signatures are low-copy transcripts, indicating that SBS is a powerful method for identifying rare transcripts [ 43].
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