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As in print publication, published data need not be free or legally unencumbered, and data use agreements constrain many published datasets.
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This is especially the case of many of the published datasets, which include samples of less than 20 individuals.
In addition, it is important to note that many of the published datasets on risk factors are based on 'relative' rather than an accurate 'absolute' risk calculation.
The fact that Pyicos performs well with simulated replicated data presents the advantage of making possible to analyse many of the published datasets that have been produced without replica.
Motivation: The continued progress in developing technological platforms, availability of many published experimental datasets, as well as different statistical methods to analyze those data have allowed approaching the same research question using various methods simultaneously.
In fact, we found that this uncovered gap problem was even more serious in many published prokaryotic datasets we have reanalyzed, a typical example from [ 60] is shown in Additional file 1: Figure S4.
Antisense ncRNAs exist for many S. cerevisiae genes; combining published datasets we found that 15 30% of yeast open reading frames (ORFs) have an annotated antisense ncRNA depending on the minimum size of overlap considered (Xu et al., 2009; van Dijk et al., 2011), not including overlapping convergent ORFs.
The comparative analysis of recently published datasets involving human gene expression responses to two bacterial respiratory pathogens illustrates many of these points.
Two published datasets were used.
For previously published datasets, we ask authors to cite both the related research articles and the actual datasets.
I.F.K., C.N.Y., J.M.C., J.S. and J.S.P. analysed data from genome-wide experiments and from published datasets.
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