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This substrate should facilitate investigating the behavior of many neuronal types on electrospun fibers in serum-free conditions.
Dendritic arbors of many neuronal types in both vertebrates and invertebrates exhibit self-avoidance and S/NSD (references in Introduction).
Dendritic and axonal arbors of many neuronal types exhibit self-avoidance, in which branches repel each other.
In many neuronal types, autapses cannot form because pre- and postsynaptic machinery are confined to axons and dendrites, respectively, which are physically segregated.
The ease of fabrication and cell culture, as well as the coupling to MS, make this device readily amenable to many neuronal types.
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For example, genetic data from C. elegans indicate that Slits might function through DCC (Deleted in colorectal cancer) (Yu et al., 2002), a molecule found to be important in the migration of many neuronal cell types, including GnRH neurons.
As an ECM protein, collagen type I supports the attachment and growth of cells, particularly many neuronal cell types.
The PI3K-Akt signaling pathway is capable of blocking cell death and promoting cell survival of many neuronal cell types [55].
The neuropeptide vasoactive intestinal polypeptide (VIP) is synthesized by neurons in the ventral aspect of the SCN [6], [7], while its cognate receptor, VPAC2, is expressed by many neuronal cell types in this structure [8], [9].
A recent study suggests that HDAC2 is produced in many neuronal cell types and in oligodendrocytes, but not in astro- or microglia (Yao et al, 2013).
The temporal resolution of this approach can be limited as many caged compounds act for long periods once released [ 7] and selectivity is often poor since common transmitters act on many neuronal cell types [ 1].
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many neuronal systems
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