Sequencing of dissected mutated ovaries showed many mutations at the designed targeting site.
To obtain insight into regulatory mechanisms of TRPV4, we focused on TRPV4-ARD, which can mediate channel regulation through binding of ligands to its concave surface and is the target of many mutations causing human diseases.
Hence, any given protein is not many mutations away from acquiring or losing a given targeting signal.
The earlier research has uncovered many mutations and led to the development of a few so-called targeted drugs, which treat some cancers by homing in on specific defects in the cells.
"Many mutations in the mitochondria gradually dis-able the cell's energy production".
Many mutations to nondetectability occurred.
This discovery also revealed that de facto, an unplanned international experiment in the mouse has been going on for 3 decades, in which many targeted mutations on C57BL/6J background have unintentionally been combined pairwise with a pleiotropic mutation, often resulting in synthetic lethality.
On top of this, Dr Huang found that there were many "off-target" mutations in other parts of HBB, or in other, similar genes, which would have caused serious damage if expressed in an actual human being.On the face of things, then, the experiment did not work.
Novel mutations target distinct subgroups of medulloblastoma.
