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Many mutants with a functional loss of β-oxidation that thereby diminishes seed germination or seedling growth have been studied.
The high mutational heritability could be due to a few mutants of large effect, or many mutants with smaller effects.
This result suggests that many mutants with lower lifespan scores can feature longer CLS compared with wild-type.
Surprisingly, however, many mutants with small birth size did not have a small size overall (ρ = 0.40; see Figure 3).
Among these were many mutants with altered surface glycosylation, which have been extensively described (bus and srf mutants [ 16 20]).
Many mutants with small birth size also had normal %G1 DNA content (see Figure 5, and File S1).
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In an attempt to screen for the phiC31 mutants that specifically recognized and integrated into target sites more efficiently, the technique of DNA shuffling was employed, and it had obtained many mutants, which with increased integration efficiency in human cells [36], [37].
In order to test the LFCH, one has to construct as many mutants as possible with the following properties: 1.
However, the MMS Rnr3 R-SGA screen had twice as many mutants in common with the MMS sensitivity screen as did the UT Rnr3 R-SGA screen.
However, many mutants for genes with pivotal functions as unfolded protein response targets could not be assayed because they lacked homokaryotic mutants, such as grp-78 (NCU03982) and pdi-1 (NCU09223), as well as nearly half of the genes engaged in protein translocation, glycosylation, and trafficking (Additional file 3: Table S3 p3).
Rather than choosing one of the many mutants in the library with a transposon inserted in a particular gene, we corroborated behavior observed during the selections using strains where the gene of interest had either been replaced with a kanamycin (kan) resistance cassette or removed to create an in-frame deletion.
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