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Many more modifications are observed in 135K/25Na/50HEPES/0Mg, however, particularly for nucleotides 89−96, for J11/12 and J12/11, and for the GAAA tetraloop.
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Of course, the combination of additional enrichment steps for specific subproteomes would presumably have resulted in the identification of many more modification sites, but the incorporation of an additional enrichment step would have made difficult the simultaneous analysis of unmodified peptides.
Many more such modifications may exist and it will be interesting to examine if any are affected in CIN tumor cells.
Many more are in trial modifications with an uncertain fate.
And future progress reports will no doubt show many more homeowners receiving big loan modifications.
Apparently, this is not sufficient for more complex organisms to adapt to the daily cycle, and thus specific cis-elements evolved, along with many more transcription factors and various posttranslational modifications of the chromatin to modulate rhythmic expression of CCGs.
The sheer number of novel modifications begs the question how many more types of PTMs are there remaining to be found?
Nowadays, the so-called omics technologies allow for inclusion of many more data sets, e.g. protein phosphorylation or epigenetic modifications.
Not surprisingly given the modifications we made our method discovered many more peaks than the original Paraclu.
More than 100 distinct histone post-translational modifications have been identified thus far, and likely many more have not been uncovered yet [ 16].
If patients with a poor early response were identified, then modification could be taken at an early stage, before many more cycles of ineffective therapy were delivered.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com