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Certain decisions as to how many microarray slides will be used and which mRNA samples will be hybridized to each slide must be made in preparation of mRNA samples before carrying out a microarray experiment [ 6, 7].
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Earlier work has shown the presence of contaminant fluorescence in the green channel of many common types of microarray slides [ 17].
↑: up-regulated; ↓: down-regulated; * p < 0.05; **: p < 0.01 As mentioned earlier many of the sequences in our microarray slides were unknowns.
The database allows for the correlation of expression levels and function by linking the EST data with the microarray results, since many of the ESTs were printed on the microarray slides.
The microarray slides were custom-designed and contained 101,720, 60 mer probes.
RNA from E14.5 embryonic hearts was isolated and then blotted with microarray slides.
Transcriptomic raw data were obtained by scanning of the microarray slides using Illumina GenomeStudio V2010.3.
Peptide microarray slides usually contain positive control spots to gauge for antibody binding.
Hybridization and washing of microarray slides (GPL7252, 43494 probes [Edgar et al. [2002]) were performed following manufacturer's protocols.
We thank PFGRC for providing microarray slides.
The microarray slides were scanned using ScanArray Express.
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