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Actually, we made many isolates (a total of 48) between 1983 and early 1984.
As TYLCV continues to spread, many isolates have been described in different parts of the world.
The many isolates of the new viruses analyzed in this report showed little variation among them.
Initial studies indicated many isolates were able to grow and exhibit excellent surface activity when supplemented with Manipueira medium.
On analysis many isolates were classified as separate entities on the basis of DNA co-migration only.
The presence of even only one R gene can induce resistance to many isolates of M. oryzae (Qu et al. 2006).
The test did not amplify A. centrale or A. ovis, and discriminated A. marginale by amplifying repeat regions within the msp1α gene which vary in number between many isolates.
Many isolates can be connected to others by means of multiple evolutionary paths.
As in other CC45 strains, egc is present but seg is not detectable in many isolates.
However, while many isolates of the most notoriously virulent serotypes (M1 and M3) carry speA, others do not [18].
Many isolates, such as race 7 possess Avh72 and Avh275c but lack copies of Avr1a (Figure 2C and Figure 6B).
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