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Exact(3)
The level of Nanos3 mRNA in somatic tissue from E9.5 to E13.5 was maintained at very low levels compared with the gonads which containing many germ cells (Fig. 4C).
However, immunostaining analyses with an antibody against mouse vasa homologue (MVH), a marker for germ cells, revealed no MVH-positive cells in Nanos2-null male gonads with NANOS2-ZM at E18.5 (Fig. 3I), although many germ cells were present in the embryonic testes of Nanos2−/− mice (Fig. 3G,H).
In that period, there is a hormonal surge (Forest et al. 1973; Quigley 2002), defined as "neonatal minipuberty," in which an increase of follicle-stimulating hormone (FSH) and inhibin B (Andersson et al. 1998) induces and reflects a strong proliferation of Sertoli cells (Cortes et al. 1987), the number of which determines how many germ cells can develop into spermatozoa (Sharpe et al. 2003).
Similar(57)
Many germ cell promoters are hypomethylated when active in germ cells and hypermethylated when silenced in somatic cells [24], [26], [27].
The expression of germ markers at the undifferentiated stage was not surprising given recent reports that many "germ cell-specific" genes are also expressed in pluripotent stem cells [13], [16], [26], [27].
Encroachment of H3K27me3 into germ cell-expressed loci could result in repression of many germ cell-essential loci.
Even with the comprehensive annotation coverage in our analysis, we expected many germ cell specific lncRNAs would be missed.
Expression of lag-2p:: inx-8:: gfp was detected in the DTC cell body as well as long processes that extended over many germ cell diameters.
We reasoned that epigenomic features specific to germ cells can be found by focusing on the X chromosome, because the X chromosome carries many germ cell-expressed genes and undergoes major epigenetic changes (e.g. X chromosome reactivation) during germ cell development.
Thus, the functional BTB, restricts the permeability of many foreign compounds to male germ cells.
The observation that transcriptional elongation is linked to DNA methylation has been noted in many contexts in addition to male germ cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com