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We further performed an exploratory miRNA expression analysis of 26 paired pre- and post-chemotherapy specimens and we observed many expression changes following exposure to chemotherapy (70 miRNAs differentially expressed), which were non-overlapping with the predictive or prognostic profiles described above.
Notably, many expression changes are evident even at a relatively young adult age, suggesting that aging is a continuous process set in motion long before phenotypic changes are detected.
Simultaneous deletion, on the other hand, results in slower growth and many expression changes (RGR rgm1Δ usv1Δ = 0.89; Fig. 4a).
We found many expression changes associated with sex and thus investigated if the X chromosome was enriched for differentially expressed genes or splicing events.
Whereas deletion of GZF3 has no effect on growth and expression (RGR gzf3Δ = 1.02; Fig. 1b, left panel), deletion of GLN3 results in a growth defect and many expression changes (RGR gln3Δ = 0.8; Fig. 1b, middle panel).
Deletion of either ECM22 or UPC2 does not affect growth and neither induces many expression changes (RGR ecm22Δ = 1 and RGR upc2Δ = 1.03; Fig. 1a, left and middle panel).
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The color coded presentation of profiles revealed that many gene expression changes upon aging are also differentially expressed upon serial passaging in vitro (figure 2).
Only a subset of the 490 differentially expressed genes are likely to be direct targets of FOXO1a, since many gene expression changes likely result from regulatory network perturbations (e.g., the genes may be regulated by the direct targets of FOXO1a, or by genes that are farther downstream in the cascade.
Many gene expression changes in the tumor compartment were thus linked to interferon signaling pathways [ 88].
Thus, as has been described in anurans, many gene expression changes in axolotl are transient, increasing initially and then decreasing.
Despite the global correlation between gene expression and copy number, many individual expression changes could not be explained by structural alterations.
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