NGS has led to a huge amount of sequencing data which has rendered many existing sequence alignment tools obsolete.
Many existing sequence data sets that characterize viral populations were generated using the Roche 454 technology.
Looking at the events, it turns out that in many cases no novel sequences actually were gained but existing sequences converted to introns.
Given this flexibility of the read simulator, it is possible to construct many kinds of individual fragments like, for example reads, contigs or expressed sequence tags (ESTs) derived from existing sequences.
Sequences with identity <97% to the existing sequences in the database were considered novel.
Furthermore, it is insufficient to use existing automated cross-walk algorithms, because many existing ICD-9 coding sequences have more than one corresponding coding section in ICD-10.
We initially thought that the weightage of gene context similarity score should be much lower that of the sequence similarity as many existing methods make use of sequence similarity but not gene context similarity.
Many databases have been created to describe orthologous genes based on existing sequence data.
EST projects on metazoa, with relatively close ancestry to many genomic model organisms, can yield high proportions of ESTs (e.g. > 95%) that are identified by reference to existing sequence databases (e.g. [ 50]).
These analyses highlight variable regions based on existing sequence data.
Many existing work depends on a secondary step of sequence motif analysis for locating binding sites in detected binding regions.
