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Bovine polyomaviruses were also detected and quantified in slaughterhouse wastewater and river waters, which shows the spread of these viruses in many environmental samples containing contamination of bovine origin.
Similarly, no fungal isolations could be made from many environmental samples that had been collected from various sites.
The species coexist in nature and co-occur in many environmental samples.
Many environmental samples from wet markets have been contaminated with the H5N1 virus, suggesting that indirect contact can be a mode of transmission of H5N1 virus.
S. schenckii (clinical strain) was not isolated from any of the control hay samples, although other members of the Opiostoma Sporothrix complex were isolated in many environmental samples.
Since the usual high richness of Gammaproteobacteria and Bacteroidetes in many environmental samples was found, it is noteworthy whether they were overestimated by the inefficient DNA extraction to some extent.
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Like many other environmental samples, extracts from composted biomass materials may contain high concentrations of organic matter.
The high reliability and sensitivity of the qPCR assay described here make it very useful for screening of samples containing few organisms and potential inhibitors, as is the case in many environmental and clinical samples.
To understand the overall fungal diversity and related ecology in some environmental samples, many ecologists would be satisfied with a tree-based genus-level estimation of fungal diversity, which would provide the likely ecology and biology of the unknown lineages.
When more discriminatory methods based on detection of variable number tandem repeats were used, many IS 2404-positive environmental samples were reported to lack M. ulcerans (18 ).
Over the past few years, cultivation-independent identification of microbial organisms by PCR amplification and sequencing of small-subunit ribosomal RNA SSUU rRNA) genes revealed a huge diversity of eubacterial and archaeal phylotypes in environmental samples, many of which are not represented by cultured organisms [ 1, 2].
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