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Beyond the separation of the mRNA the preparation of amplified cDNA as well as WGA DNA involves many discrete steps which are prone for technical variation, such as the magnetic coupling and binding of mRNA to the column, the elution of DNA from the column, the PCR amplification of the double-stranded cDNA and library preparation which adds another step of PCR amplification.
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A cellular automaton is a lattice of cells; each cell can take one out of finitely many states and updates its state in discrete steps depending on the state of its neighboring cells.
Moreover, many continuous events are conceived of as sequences of discrete steps.
To address that, his team broke down the process of cardiomyocyte differentiation into discrete steps, then isolated and controlled as many variables as possible.
Analyzing the measured extension changes from many measurements reveals that the SSB is unraveled in discrete steps, corresponding to the same pathway of intermediates, (SSB 35 → (SSB 17 → unbound, as proposed above.
The domain formed a 1,62 × 1,685 computational network comprising many discrete 400 m × 400 m grid cells, with each time step of 0.58 s and totally 26,001 computing steps, about 16,000 s or 4.5 h.
Therefore, proper synaptogenesis probably requires more than just one 'master molecule', with many molecules functioning instead, either separately or in cooperation, in the discrete steps of synapse formation.
The iMac Pro goes one step further and uses the T2 to replace many discrete controllers.
Poems 1960-2000 gathered Fleur Adcock's many discrete volumes into a hamper.
The egg-based vaccine depends on six discrete steps, and takes at least six months, or longer, to produce.
Too many discrete songs, one after the other, is like reading a joke book or eating candies.
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