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A good RNA isolation protocol is of high importance, since RNA is more unstable than DNA and many clinical samples contain RNAses.
Established technologies are available to carry out global transcriptomics using nanograms of input RNA, however, many clinical samples of low cell content would be expected to yield RNA within the picogram range.
Stem and progenitor cells from tissue niches may be found in many clinical samples.
Some of these assays require micrograms of DNA which may be difficult to obtain for many clinical samples.
Necessary clinical samples were taken, entirely independent of how many clinical samples were processed for microbiological analysis.
Small sample analysis of expressed proteome is critical for many clinical samples since they represent points in time for disease progression in individual patients.
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However, the sensitivity of Sanger sequencing is suboptimal for many clinical tumor samples.
Further, the protocol applied for the assay was the same as for using the in situ PLA on ethanol fixed cells, i.e. not optimized, but the observation of signals is an important step in the development since it opens up for fast investigation of many clinical tumor samples available on TMAs in the future.
The MSE method can be applied to analysis of DNA methylation status in many different clinical samples, and this may facilitate identification of new risk markers.
Thus, MSE can be applied to analysis of DNA methylation in many different clinical samples, and this may facilitate identification of new risk markers.
As reported previously, the long PCR products required for our MitoChip protocol could not be obtained from many of the clinical samples [ 50].
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